In addition, AFLP can be useful for studying genetic variation in different yeast species used in wine making, “Amplified fragment length polymorphism (AFLP) was used to investigate genetic variation in commercial strains, type strains and winery isolates from a number of yeast species” (Lopes et al). AFLP can also be applied to criminal investigations and paternity testing. Besides detecting fragment polymorphisms, “The AFLP technique may be used for fingerprinting of cloned DNA segments like cosmids, P1 clones, bacterial artificial chromosomes or yeast artificial chromosomes (Vos et al). “For a wide range of taxa, including plants, fungi, animals and bacteria, AFLP markers have been used to uncover cryptic genetic variation of strains, or closely related species, that had been impossible to resolve with morphological or other molecular systematic characters” (Mueller et al). Further more, AFLP markers are very useful for studying genetic variation between closely related species. One of the key features of AFLP analysis is the detection of polymorphisms of genomic DNA fragments obtained through restriction digestion. And the third step is the analysis of the amplified fragments after visualization on a denaturing polyacrylamide gel.ĪFLP is a powerful molecular tool allowing simultaneous screening of a large number of DNA regions that are spread randomly throughout the genome. The second step consists of the amplification of certain restriction fragments with PCR primers (the primers are designed to target the adapters fused to the end of the fragments). There are several reasons for the use of two types restriction enzymes: generation of short DNA segments with good amplification and separation on the gel, the rare cutter will complement the frequent cutter by decreasing the number of generated fragments, and only one strand of the double stranded PCR products can be labeled. The first step involves restriction digestion of DNA with two types of enzymes (a rare and a frequent cutter), followed by the ligation of double-stranded oligonucleotide adapters, specific to the ends of the generated fragments. This PCR based tool utilizes restriction enzymes to digest genomic DNA to smaller fragments and consists of three main steps. AFLP is a DNA fingerprinting technique that can be used to answer a variety of research questions.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |